Dichotomous key to European Hypoxylon

           

Illustration of characters
Ostioles papillate, opening higher than the stromatal surface A
Ostioles umbilicate, opening lower than or at the same level as the stromatal surface B
 
A- Ostioles encircled by a conspicuous flattened to slightly concave disc A1
A- Ostioles not encircled by a disc A2
 
A1- Perithecial mounds inconspicuous. Ascospores 6-8 x 3-3.5 µm. H. stygium var. annulatum
A1- Perithecial mounds conspicuous. Ascospores 11-15 x 4.5-5.5 µm H. michelianum
 
A2- Perithecia small, 200-300 (400) µm diam, the tissue below the perithecial layer weakly developed, < 0.3 mm thick; stromata effused to pulvinate A3
A2- Perithecia larger, 400-600 µm diam, the tissue below the perithecial layer 0.5-3.5 mm thick; stromata pulvinate to peltate, often constricted at base, often coalescent A7
 
A3- Mature stromata carbonaceous, black , without KOH-extractable pigments (stromata orange with violet pigments when immature) H. submonticulosum
A3- Mature stromata waxy, with KOH-extractable pigments A4
 
A4- Stromata with orange brown tones, umber (9), rust (39) or sepia (63), with KOH-extractable pigments orange (7); ascospores averaging less than 20 µm long A5
A4- Stromata with purplish tones, vinaceous purple (101) to brown vinaceous (84), with KOH-extractable pigments olivaceous (45), isabelline (65), livid violet (79) or absent; ascospores averaging more than 20 µm long A6
 
A5- Ascospores averaging more than 15 µm long H. julianii
A5- Ascospores averaging less than 11 µm long H. rutilum
 
A6- KOH-extractable pigments olivaceous (45) to isabelline (65), ascospores 22-31 x 8.5-11 with faint germ slit less than spore-length H. macrosporum
A6- KOH-extractable pigments livid violet (79), pale purplish grey (127) or absent, ascospores 18.5-23 x 8-10 µm with germ slit spore-length H. vogesiacum
 
A7- KOH-extractable pigments orange (7) to rust (39), stromata small-sized, pulvinate, erumpent, restricted to Populus sp. H. laschii
A7- KOH-extractable different A8
 
A8- Ascospores 6.5-8.5 x 3-4 µm; stromata growing on Quercus and Castanea H. cohaerens var. microsporum
A8- Ascospores larger than above; on different hosts A9
 
A9- Apparently restricted to Fagus. Ascospores 9-11 x 4-5 µm with germ slit spore-length H. cohaerens
A9- Growing mainly on Alnus, Betula and Corylus. Ascospores 8.5-12 x 3.5-5 µm with germ slit less than spore-length H. multiforme

           

B- Stromata hemispherical to almost spherical B1
B- Stromata effused to pulvinate B4
 
B1- Stromatal surface greyish sepia (106) to dark brick (60). KOH-extractable pigments greenish yellow (16), ascospores 17-22 x 9-11 µm H. intermedium
B1- Stromatal surface rust (39), bay (6) or dark brick (60); KOH-extractable pigments orange (7), ascospores averaging less than 15 µm long B2
 
B2- Ascal apical ring absent H. commutatum
B2- Ascal apical ring present, amyloid B3
 
B3- Stromata growing mainly on Fagus. Ascospores 11-13.5 x 5-6.5 µm H. fragiforme
B3- Stromata growing on other hosts, rarely on Fagus. Ascospores 7-9 x 3.5-5 µm H. howeianum
 
B4- Stromatal surface with vinaceous purple (101) to dark vinaceous (82) tones B5
B4- Stromatal surface with orange (7), rust (39), brick (60), or sepia (63) tones B12
 
B5- Ascospores averaging more than 20 µm long B6
B5- Ascospores averaging less than 15 µm long B7
 
B6- KOH-extractable pigments livid violet (79), pale purplish grey (127) or absent, ascospores 18.5-23 x 8-10 µm with germ slit spore-length H. vogesiacum
B6- KOH-extractable pigments olivaceous (45) to isabelline (65), ascospores 22-31 x 8.5-11 with faint germ slit less than spore-length H. macrosporum
 
B7- KOH-extractable pigments dilute livid violet (79). Ascospores 8-11.5 x 4.5-5 µm H. carneum
B7- KOH-extractable pigments orange (7). Ascospores 9-11.5 x 4.8-6 µm. H. petriniae
B7- KOH-extractable pigments amber (47) to olivaceous (48), with yellow green or brown tones B8
 
B8- Stromatal surface with a metallic shine when mature; perithecia obovoid to tubular, up to 1000µm high. H. macrocarpum
B8- Stromatal surface lacking a metallic shine when mature; perithecia spherical to obovoid, small-sized, not tubular B9
 
B9- Ascospores with straight germ slit. B10
B9- Ascospores frequently with a sigmoid germ slit. B11
 
B10- KOH-extractable pigments amber (47) to ochreous (44) or greenish yellow (16); ascospores 9-12 x 4.5-6 µm, ellipsoid-inequilateral, with a dehiscent perispore H. perforatum
B10- KOH-extractable pigments olivaceous (48) to grey olivaceous (107); ascospores 12-15 x 5-7 µm, ellipsoid-equilateral often pyriform, with indehiscent perispore H. fuscopurpureum
 
B11- Stromata with bright yellow granules, KOH-extractable pigments isabelline (65) to grey olivaceous (107); restricted to Quercus, with a boreal distribution. Ascospores 10-13.5 x 4-5 µm H. porphyreum
B11- Stromata with dull yellow granules, KOH-extractable pigments amber (47), luteous (12), citrine (13), olivaceous (48); plurivorous and widespread, but not recorded on Quercus. Ascospores 11-16 x 5-8 µm H.fuscum
 
B12- Young stromata with a bright yellow to orange fimbriate margin. Perithecia small-sized, 100-300 µm diam, on a well developed black basal tissue B13
B12- Young stromata without a bright yellow to orange fimbriate margin B14
 
B13- Ascospores 5-7 x 2.5-3.5 µm, ellipsoid-inequilateral H. ticinense
B13- Ascospores 8-11 x 4-5 µm, ellipsoid-equilateral H. subticinense
 
B14- KOH-extractable pigments orange (7) to sienna (8) B15
B14- KOH-extractable pigments different, luteous (12), amber (47), greyish sepia (106), hazel (88) B23
 
B15- Ascal apical ring highly reduced or absent, inamyloid B16
B15- Ascal apical ring present, amyloid B17
 
B16- Stromata discoid, encircled with a swollen stellate margin, on bark of Fraxinus. Ascospores 9.5-12 x 5-6 µm H. cercidicolum
B16- Stromata pulvinate to hemispherical, known on bark of Carpinus. Ascospores 9-11 x 4.5-5.5 µm H. commutatum
 
B17- Ascospores averaging more than 14 µm long B18
B17- Ascospores averaging less than 12 µm long B19
 
B18- Stromata pulvinate restricted at base, with rust brown to ochraceous brown granules beneath surface. Growing on Tilia. Ascospores 14-17 x 6.5-8 µm H. ferrugineum
B18- Stromata effused to pulvinate, with red granules beneath surface. Growing on various hosts. Ascospores 15-18 x 6-7.5 µm H. julianii
 
B19- Perithecia 0.1-0.35 mm diam. Ascospores 7-10 x 3-4.5 µm H. salicicola
B19- Perithecia averaging more than 0.4 mm diam B20
 
B20- Stromata erumpent, pulvinate, small-sized, up to 6 mm diam. Growing on Populus. Ascospores 8-10 x 3.5-4.5 µm H. laschii
B20- Stromata non erumpent, effused to pulvinate, larger. Ascospores larger B21
 
B21- KOH-extractable pigments orange (7) to rust (39) or sienna (8), ascospores brown, ellipsoid-inequilateral; widespread and plurivorous B22
B21- KOH-extractable pigments usually luteous (12) hazel (88), sometimes sienna (8); ascospores dark brown, nearly equilateral, 9.5-12.5 x 4.8-6 µm; restricted to Sorbus, with a boreal distribution H. liviae
 
B22-Ascospores 9-13 x 4-5.5 µm. Germ slit straight. H. rubiginosum
B22- Ascospores 11.5-14.5 x 6-7 µm. Germ slit sigmoid H. crocopeplum
 
B23- Ascospores dark brown, nearly equilateral; restricted to Sorbus, with a boreal distribution; ascospores 9.5-12.5 x 4.8-6 µm H. liviae
B23- Ascospores medium brown, inequilateral; plurivorous B24
 
B24- KOH-extractable pigments amber (47) with greenish-yellow tone, ostioles surrounded by a conspicuous white material; perithecia obovoid, 300 µm diam H. perforatum
B24- KOH-extractable pigments greyish sepia (106) to umber (9), ostioles lacking white material, perithecia usually tubular, 500 µm diam, up to 1 mm high H. macrocarpum